Outlines
Stochastic Nature of Single Molecules
Fluctuation Measurements by Fluorescence Spectroscopy
From Discrete Markov Model to Continuous Diffusion
Brownian Motion (Diffusion) in a Continuous Energy Landscape
Quantitative Equation of Motion
Smoluchowski Equation, Markov Process & Chemical Kinetics
Protein-Ligand Separation with AFM
Energy Landscapes in Forced Protein-Ligand Separation
Hysteresis in Macromolecular Mechanics
Stochastic Barrier Crossing and Brownian Dynamics
What one learns from P(x,t)?
Stochastic Forced Separation
Equilibrium and Nonequilibrium Steady-State, Cycles & Pumping
Stochastic Michaelis-Menten Kinetics of Single Enzymes
Oscillatory Enzyme Kinetics
The implication of an oscillatory enzyme reaction:
Internal & External of a Motor Protein
Thermal Equilibrium & Cycles
How motor protein generate force?
Free Energy Landscape of Motor Proteins:the “Power Stroke” and the “Ratchet”
From Motor Proteins to Muscle Contraction: Sliding Filaments and Cross-bridges
Two Different Classes of Models on Cross-bridge Kinetics
Microscopic and macroscopic views of force generation
Duality: Transition vs. State Views
Summary
Summary (Continued)
Molecular Biophysics at Three Different Levels of Approach
Email: qian@amath.washington.edu
Home Page: http://www.amath.washington.edu/~qian
